Immunofluorescence Analysis of Human Endocervical Tissue Explants Infected with Neisseria gonorrhoeae
نویسندگان
چکیده
منابع مشابه
Ultrastructural analysis of primary human urethral epithelial cell cultures infected with Neisseria gonorrhoeae.
In men with gonococcal urethritis, the urethral epithelial cell is a site of infection. To study the pathogenesis of gonorrhea in this cell type, we have developed a method to culture primary human urethral epithelial cells obtained at the time of urologic surgery. Fluorescent analysis demonstrated that 100% of the cells stained for keratin. Microscopic analyses indicated that these epithelial ...
متن کاملImmunofluorescence staining for the detection of Neisseria gonorrhoeae in women.
Although the use of a fluorescent antibody (FA) staining technique to demonstrate N. gonorrhoeae in secretions from patients with gonorrhoea was first described as long ago as 1959 by Deacon, Peacock, Freeman, and Harris, it does not seem to have gained wide acceptance in routine diagnosis. In our laboratory direct and delayed FA methods were first assessed in 1963 (Fry and Wilkinson, 1964). In...
متن کاملNeisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants
BACKGROUND A role for pilus during attachment of Neisseria gonorrhoeae to epithelia of the female reproductive tract is currently assumed. However, Pil⁻ gonococci have been observed during infection of the reproductive tract, which prompted us to examine the effect of pili on the dynamics of infection and the inflammatory responses of mucosal explants of the human fallopian tube. METHODS Muco...
متن کاملComparison of direct inoculation and Copan transport systems for isolation of Neisseria gonorrhoeae from endocervical specimens.
Two commercial swab transport systems, Copan Amies gel agar with and without charcoal (Copan Diagnostics, Corona, Calif.), were compared to direct inoculation onto modified Thayer-Martin medium for detection of Neisseria gonorrhoeae in 1,490 endocervical specimens obtained from women attending a sexually transmitted disease clinic. Copan swabs were held in the transport system for 24 h at room ...
متن کاملSoluble pilin of Neisseria gonorrhoeae interacts with human target cells and tissue.
Pili of Neisseria gonorrhoeae are phase-variable surface structures that mediate adherence to host target cells. Each pilus is composed of thousands of major pilus subunits, pilins, pilus-associated protein PilC, and possibly other components. Piliated and nonpiliated gonococcal clones may secrete a soluble smaller pilin (S-pilin) that is cleaved after amino acid 39 of the mature pilin protein....
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2018
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2720